Empower Tip: Review Window and the Processing Method

Let’s start from the Curves tab in Browse Project. The current calibration has ID 6240 and the older calibration has ID 6213. Let’s first look at the difference between them using the Compare window. ( You may recall we covered that in Tip 8, Comparing Calibration Curves )

In the Compare Window with Calibration ID 6240 highlighted, click the Individual Points tab.

The X Values for the 3 levels are 0.02, 0.05 and 0.1 respectively.

The older Calibration ID of 6213 has X Values which are slightly lower due to a Purity factor that was applied. Our goal is to use this older calibration curve where the Purity factor had been applied.

Let’s now bring a Result Set or a Result into Review and click the Calibration Curve tool.

In the Calibration Curve Window go to Options, Select Calibration.

Select the Calibration Curve you want to use (in this case Calibration ID 6213) and click OK.

Click OK to the message which says the calibration you have selected is not the most current.

Calibration curves are associated with the processing method so from the File menu select Save As, Processing Method to give the method a different name.

You will be asked to Clear Curves or Copy Curves. Select Copy Curves.

Enter a new name for the Processing Method and click Save.

Exit from Review and now process the Sample Set using the older calibration curve. Go to the Sample Sets tab in Browse Project, right click on the Sample Set and select Process.

In the Background Processing and Reporting dialogue box select the Processing Method with the different name, and from the 'How' drop down menu select Quantitate Only. Then click OK.

Go to the Result Sets tab, right click on the new Result Set and select Review.

Click the Calibration Curve tool.

The X Values for Uracil are from the older calibration curve.

If we compare the results for a sample we see a different calculated amount for Uracil when we use the older calibration curve. Want to know how to create this report? Stay tuned for future tips on Report Methods.

It’s that easy!