Empower Tip: Dissolution Option in Empower

In this tip, we will discuss the Dissolution Fit Factors f1 and f2.

Dissolution Fit Factors f1 and f2 are response variables in statistically designed experiments. These fit factors can tell us if the dissolution data between two sample sets and profiles are fit.

Calculating f1 and f2 between two sets of dissolution runs is simple with some pre-created custom fields. These custom fields are available to be copied to your Empower dissolution project from the Dissolution Default project. If you do not have the Dissolution Default project, it can be downloaded from the Waters Marketplace. Login at https://marketplace.waters.com.

To properly calculate f1 and f2, only two sample sets should be found in the project. In this way, only two result sets should exist in this project. This also means that the data should be collected as sets and using the Dissolution Sample Set Method wizard.

f1 represents the percent difference between the dissolution curves at each time point. Acceptable f1 values are between 0 and 15. Results close to 0 are best as these represent dissolution values that are statistically close to each other.

f2 represents the percent similarity between two dissolution curves. F2 values between 50 and 100 suggest that the two dissolution profiles are similar.

Custom fields for f1 and f2 are available in the Dissolution Default project.

In the custom fields, the channel name of “2487Channel 1” was hard-coded in. It is required to modify the channel name in each formula to the correct channel name. If there is only one channel, it is best to remove the channel name from the formula, however allowing the syntax to be properly used. The following example shows the channel to be nullified in the syntax of the custom field.

Labels for injections for calculations should match custom fields. For example, TIME10, TIME20 and TIME30 were used in the formula. Also, the six or however many vessels per bath that were collected from the same time point should have matching labels to allow average calculations to be calculated properly.

Also, depending on how many time points, the custom fields need to be expanded to include all time points. In the example, custom fields, three time points were included for the Sums calculations.

When processing data, if the Sample Sets are labeled “SS1” and “SS2”, select SS2 and process.

NOTE: at this time, f1 and f2 will not be calculated. Then, select SS1 and process. SS1 will have the proper f1 and f2 calculations.

It’s that easy!