waters_connect 3.2.0 LTS Release Overview

waters_connect Base Kit comprises of platform services, utility tools and core applications such as Hub, Administration, System Console, Device Management, Explorer, Qualification for general system use.

Key benefits in this release include:

• Elevate your product understanding when using the waters_connect for Quan and the Biologics workflows with the software translation in Japanese.
• Increased compliance readiness with more comprehensive insights in the audit trail for acquisition events and acquisition method, such as:
  • advanced user setting restrictions for customizable reports,
  • better traceability in the audit trail for records (such as methods, experimental records, post run reports and instrument details) associated to a data set,
  • view records in draft within the Explorer application.
• Improve your lab productivity and reduce instrument cost of ownership by connecting up to 5 Xevo Tandem Quadrupole instruments in a single network deployment.
• Extend the capabilities of your instrument systems inlet and sources with the support for multidimensional liquid chromatography with ACQUITY UPLC System with 2D LC Technology for accurate mass workflows within the UNIFI application.
• Improved product robustness and stability when acquiring with the Laboratory Network Device (LND) application reduce data transfer communication bottlenecks caused by instrument errors.
• Improved performance and reliability of waters_connect Network deployments with the release of a new waters_connect LND PC.
• Improved robustness and performance when importing MassLynx data.
• Increased usability and productivity for the waters_connect acquisition workflow with the Device Management application. This enables you to manage your laboratory network devices, instrument systems, clients and the instrument drivers;
  • one-click set-up of systems on/offline in System Console;
  • view of diagnostic channels in Sample Submission and Toolkit applications.
• Elevated usability of the UNIFI application with user interface improvements, such as redesigned icons, more data available to fit in the vertical space of your screen, more visible scrollbars and dynamic zoom in/out adjustments for tabular data;
• Improved product quality and stability by addressing high impact software anomalies such as:
  • sample tray default to ANSI-48 Vial 2ML;
  • loading time of waters_connect applications;
  • MS Quan result set character limits & draft statuses;
  • UNIFI high energy fragment table for candidate components;
  • UNIFI DDA & MSMS data send/retrieve detection results to Scientific Library.

The LC-MS Toolkit application enables data interrogation outside of the defined workflows; provides flexibility to the software that is needed for more advanced use.

Key benefits in this release include:

• Expanded tools for basic accurate mass analysis in Toolkit such as lock mass channel, combine to view spectra, Integrate, Smooth, Mass Measure, Spectra Background Subtract, Chromatograms Combine and Subtract, improved zoom functionality, and Tof/quad centroided spectra continuum data.
• The ability to create a peak list table from a chromatogram, detailing RT, Area, % Area, Height, % Height, Width, and Data Points Across the Peak
• An elemental composition calculator is included that calculates possible formulas that could account for an observed accurate mass. You can also analyze low-energy and high-energy MSe data to include a high-energy fragment in the calculation.
• Localization into Japanese.

waters_connect Acquisition Kit comprises of Sample Submission, Method Editor and Laboratory Network Device applications. This kit enables data acquisition with Waters instrument systems used accurate mass Biopharmaceutical applications with Xevo G2-XS QTof, Xevo G3 QTof and BioAccord system & the quantitation workflow with Xevo TQ-S cronos, Xevo TQ-S micro, Xevo TQ-XS and Xevo TQ Absolute. 

Key benefits in this release include:

• Extend the capabilities of your instrument systems inlet and sources with the support for ACQUITY UPLC M-Class System and NanoLockSpray Ionization Source for Xevo G3 QTof.
• Increased security with the adoption of Transport Layer Security (TLS) 1.2 for the acquisition workflow when using the Sample Submission and Acquisition Method Editor applications.
• Increased usability and productivity for the biologics workflows by automating data acquisition and processing for Xevo G2-XS QTof and Xevo G3 QTof, Intact Mass, and Peptide MAM applications.
• Increased usability and productivity for the waters_connect acquisition workflow by viewing diagnostic channels in the Sample Submission application.
• Localization into Japanese.

The MS Quan application enables routine small-molecule quantitation and accelerates quantitation data processing and review steps with exception-focused review and easy-to-view chromatogram windows for multiple samples.

Key benefits in MS Quan v1.10 release include:

• Sample factors to allow for sample dilution workflows.
• Reporting enhancements to allow users to further define the content of the PDF report.
  • Report queue to facilitate generation of multiple reports simultaneously

  • Configuration of data tables (Including/excludes columns and ordering)

  • Optional peak annotations

  • Optional axes labels

  • Extra configurable summary information

  • Visibility of all reports generated in a result set

  • Tables automatically split when too wide

  • Additional ion ratio and qual ion fields

• Localization into Japanese

• PFAS enhancements
  • R² values shown for calibrations where the user forces the origin
  • Lower bound calculations for totals by including the LLOQ value
  • An option to change the linking of Internal standards to secondary internal standards
  • Update of the retention time rule to allow identification of peaks using a relative retention time rule

Key benefits in MS Quan v1.11 release include:

• Explicit edit and discard actions on methods and results to clarify the situation around saving of data.
• The clear display of version information for all MS Quan entities. 
• MS Quan now automatically calculates a recovery % for Primary Internal Standards.
• Reporting enhancements to allow users to further define the content of the PDF report.
  • Option to set the size of chromatograms and define the axes
  • Filtering of injections/analytes included
  • Inclusion of instrument system type and name
• Improvements to the manual integration workflows that allow new peaks to be added and defined more reliably

The Peptide Multi-Attribute Method (MAM) application enables monitoring of biopharmaceutical product quality attributes (PQA) at the peptide level

Key benefits in Peptide MAM 1.8.0 release include:

• An Application Programming Interface (API) is now available that supports creation of analyses and retrieval of results by third party informatics tools.
• Enhanced processing of data containing higher mass peptides by an Improved Monoisotopic Peak Detection (IMPD) routine, which supports detection of a lower intensity or missing monoisotopic peak.
• Localization into Japanese

Key benefits in Peptide MAM 1.8.1 release include:

• Support for PDA optical detectors. In addition to TUV, users can now view the optical channels from the PDA detector.
• Support for Sample Organizer, allowing up to19 microtiter plates, or, 9 intermediate height plates, or, 6 deep-well plates. Increasing the potential throughput for Peptide MAM users.

Intact Mass enables biologics mass confirmation and purity determination of oligonucleotides, peptides, protein and conjugates.

Key benefits in this release include:

• Support for PDA optical detectors. In addition to TUV, users can now view the MaxPlot optical channel from the PDA detector, and use this channel for detection. If the injection contains PDA data, the MaxPlot chromatogram is displayed and used for peak detection. If the MaxPlot chromatogram is missing, the first optical chromatogram in the injection is displayed and used for peak detection. 
• Support for Sample Organizer and Sample Manager vials, allowing up to19 microtiter plates, or, 9 intermediate height plates (or 2-mL vial holders), or, 6 deep-well plates (or 4-mL vial holders). Increasing the potential throughput for Intact Mass users.
• Optical-only workflows with built in IEX, SEC and reverse-phase column support and analysis expand the utility of Intact Mass to groups within the laboratory without MS requirements. 
• Automatic sample report generation further supports a high-throughput Intact Mass workflow. Users do not need to open complete analyses to download individual reports. They now have the option to download reports to a local folder automatically upon completion of processing, and also the option of a high-throughput report template designed to deliver the important details from a large amount of data in an efficient manner.
• Grouped calculations allow streamlined IEX, SEC and reverse-phase analysis using MS data.
• Processing of analyses can now be cancelled, preventing loss of time and resources processing analyses with known issues.

The CONFIRM Sequence application enables streamlined oligonucleotide sequence confirmation and impurity characterization workflow

Key benefits in this release include:

• The user can now confirm if their chosen nucleic acid digestion strategies can theoretically produce the desired sequence coverage, using a visualization of theoretical digest sequence coverage, including calling out segments of isobaric mass ambiguity. This helps users to determine the best digestion strategy to achieve optimal sequence coverage using unique digestion products.
• Localization into Japanese

waters_connect Map Sequence enables accurate mass mapping of digested oligonucleotides.

Key benefits in this application include:

• Input of in silico predicted digestion products
• Processing of pre-acquired data to identify digestion products
• Grouped results for rapid assessment of sequence coverage
• Identification of structural isomers and sequence repeats
• Output of results for coverage map review

Quantitative analysis software available on our core Xevo tandem quadrupole family of mass spectrometers to enable laboratories to process large sample batches quickly and easily, significantly reducing the time spent on data review. Maximize your productivity with a compliant-ready workflow for analytical methodologies ranging from PFAS to genotoxic impurity analysis.

Biopharmaceutical accurate mass applications for protein-based therapeutics, vaccines, and nucleic acid-based therapeutics.

Bioprocess accurate mass applications for process (spent cell culture media) and product quality attribute monitoring including the Bioprocess Walk-Up Solution.

Accurate mass-based solutions for screening of small molecules with versatile qualitative and quantitative analysis of compounds like pesticides, drugs of abuse, metabolites, impurities, extractables, and leachables.

Operating System
Windows

Requirements

• Windows Defender must be set to Off
• OS language: English (US), Japanese (Japan).
• Windows images provided with waters_connect PCs include Windows IoT Enterprise licensing which enables extended support on the operating system end of service over the standard edition. View more information on Windows releases.

Versions Supported
Workstation PC

• Windows 10 Enterprise IoT LTSC 2021 edition (version 21H2)
• Windows 10 Pro version 22H2

Operating System
Windows

Requirements

• Windows Defender must be set to Off
• OS language: English (US), Japanese (Japan)
• Windows images provided with waters_connect PCs include Windows IoT Enterprise licensing which enables extended support on the operating system end of service over the standard edition. View more information on Windows releases.

Versions Supported