Empower Tip: Review Window and the Processing Method

Q: How do I use the average area of replicate injections of my sample to quantitate my analyte(s) of interest?

A: Normally, we quantitate the individual injections and then calculate the average amount; this is another way.

In this tip, I will show you how to use the average area of replicate injections of a sample to quantitate analyte(s) of interest (Part 16).

Create a Peak Type Custom Field, ‘data type real’ to calculate the average area of the replicate injections. This is an Intersample Custom Field with a summary function.

The equation for a line is y=mx+b. In Empower, it is y=Bx+A. If we solve for ‘x’, we get x=y-A/B. In this case, we want to use the ‘average area’ which is calculated from the first Custom Field. I would recommend referencing the ‘last injection’ of the ‘last standard’ for A and B, so that you have the final coefficients.

Now, we see the ‘average area’ for each component based on the duplicate injections of the sample and the ‘calculated amount’ based on that average.

It’s that easy!