Empower Tip: Review Window and the Processing Method

In this tip, we are going to learn how to optimize sensitivity by extracting a single mass rather than working with a TIC plot (Part 6). This is somewhat analogous to extracting a single wavelength rather than working with a MaxPlot when working with the PDA data.

STEP 1 We start by bringing a 3D MS Scan into review and applying the appropriate Method Set. The Processing Method is set up to integrate the main peaks and calculate the signal-to-noise ratio for each. (Note: the signal-to-noise ratio for Peak 6 is 13.3).

To optimize sensitivity for Peak 6, we can derive a single mass and add it to our Method Set. In this case, we derive 630.55, which is the base mass for this peak. (Refer back to Tip 56 which explains how to derive a single mass).

Alternatively, we could use the ‘Extract Choices’ drop down list, located in the lower toolbar. Select the ‘blank’ and then enter the desired mass. This will automatically be added to the channel table in the Method Set.

Integrate the chromatogram for the single mass. The signal to noise for the peak at this mass is now 334.2.

It’s that easy!