Empower Tip: Reporting SEC-MALS results

In this tip, we look at using Molecular Weight Markers in the Processing Method. This tip continues from Empower tip-of-the-week Tip 462.

Let’s see how it’s done!

There are six molecular weight markers in the Processing Method. Empower uses these markers when calculating the percent area of the distribution that has molecular weights either above or below the molecular weight marker. The molecular weights and percent area values appear in the Peak table of the Review Main window and the Results window.

The first three percent area values (%Poly > MWM#) are calculated as the percent area of the distribution with molecular weights greater than the molecular weight associated with the molecular weight marker. The last three percentage area values (%Poly < MWM#) are calculated as the percent area of the distribution with molecular weights less than the molecular weight associated with the molecular weight marker.

A Result for a mAb sample has been brought into Review. We see the Mw is 141879.

Navigating to the Results window within Review, we can view the Molecular Weight Distribution Plot.

Opening the Processing Method and going to the MALS Components tab, we see the fields for the 6 molecular weight markers.

I entered 150000 for Molecular Weight Marker 1 and 130000 for Molecular Weight Marker 4.

When the sample is reprocessed, we now see that 9.811% percent area of the distribution is above 150000 and 9.187% percent area of the distribution is below 130000.

I hope you found this tip helpful.

Final Note

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