Empower Tip: Empower User Question

In this tip for Empower Chromatography Data System (CDS), we will answer an Empower CDS user question.

Q: What is the difference between Purity (Vial) and Purity (Component)?

Great question. We discussed Purity (Vial) in Tip 13, but it is a good time to revisit.

To better understand what the different fields mean, let’s look at calibration and quantitation without use of these fields. Starting with External Standard Calibration. When you calibrate, the value displayed in the Amount field is the same as what was entered in the Component Editor for the standards.

When you quantitate, you see the calculated Amount for the components in the sample.

The Purity (Vial) field, found in the Component Editor, is where a purity value for the standard components can be entered. The allowable range is 0.001 – 150.000. In the example, a value of 99.000 has been entered for Propyl Paraben.

When you calibrate, the amount for Propyl Paraben has been adjusted from 0.02000 to 0.019800 based on the purity value of 99.000. The Purity (Component) field can be added to the Peaks table in Review to display the purity value entered in the Component Editor.

When you quantitate, you see the calculated amount for Propyl Paraben went from 0.065514 (Step 2) to 0.064859. The Purity (Component) field displays 100 for the sample components which essentially does not do anything to the calculation.

With internal standard calibration & quantitation, you may want to enter a purity value for the internal standard which goes into all standards and samples. In this example, Propyl Paraben is the internal standard.

When you calibrate, the amount for the internal standard has been adjusted based on the purity value entered in the Component Editor.

When you quantitate, the calculated amount for Ethyl Paraben is affected by the purity value for the internal standard.

It’s that easy!