Empower Tip: Review Window and the Processing Method

Often times I want to use my PDA for qualitative purposes, that is collect my data over a wavelength range and then look at the chromatogram at different wavelengths and the spectra for the various peaks. I want to do this without developing a processing method or deriving channels in a method set. There are several ways to do this and this is one simple approach.

Let’s start by bringing a 3D PDA channel into review. You can use injections or sample sets depending on your preference.

Click the Extract Chromatogram tool and a wavelength marker appears to right of the contour plot.

Using the mouse, slide the marker to the desired wavelength and the chromatogram appears in the chromatogram plot.

You can also double click inside the marker, enter a specific wavelength, and press the enter key to move to a desired wavelength.

Let’s repeat steps 2 and 3 to extract another chromatogram.

Click the overlay tool to compare the chromatograms.

Finally, click the 2D Channels tab in the lower left portion of the window to display a table listing of the extracted chromatograms.

That was easy! Now let’s look at the spectra for the peaks.

Click the Extract Spectrum tool and a marker appears along the time axis of the contour plot.

Using the mouse, drag the cursor to a peak and the spectrum is displayed in the Spectrum Review window.

You can also double click inside the marker, enter a specific time, and press the enter key to move to a desired peak.

Repeat steps 8 and 9 to extract a spectrum from another peak. The spectra are automatically overlaid in a stack plot layout.

Any overlay (chromatograms or spectra) can be modified by right clicking in the plot and selecting properties.

The overlay tab allows you to modify the overlay and the legend tab allows you to add information to the plot.

It’s that easy!