Empower Tip: Dissolution Option in Empower

In this tip, we will discuss the ways Dissolution is calculated effectively.

We begin this tip by showing you a Sample Set Method created with Dissolution Bath A selected for 6 vessels and three transfer times (60, 120, and 240 minutes) REQUIREMENT: To create sample sets for Dissolution, you must use the Sample Set Method Wizard.

Before or after acquiring data, there are limited edits you are allowed to make with Dissolution Sample Set Methods. You are not allowed to edit fields such as Bath, Vessel, or Transfer Time of the samples, especially after acquisition. Allowed edits are limited to the available tabs when selecting Edit > Dissolution and may require going through the Sample Set Method Wizard again.

One of the possible edits allowed with Dissolution Sample Set Methods is the Dissolution Wait function. This function can be inserted to allow a pause time when the samples are unavailable to be acquired. This is essential when the Sample Set is set up utilizing an automated transfer module for dissolution.

The Compute Dissolution function is automatically added at the end of a Dissolution Sample Set Method. This function occurs after results from one or both dissolution baths are quantitated but before a report is generated.

This function calculates Amount Dissolved and Percent Dissolved for each peak specified in the Components page of the Sample Set Method Wizard. The Compute Dissolution function uses the Sample Set Method parameters Media Volume, Removed Volume, Replaced Volume, Component Names, and Claimed Amounts. In addition, the Compute Dissolution function uses the Bath, Vessel, and Transfer Times defined in the Sample Set Method

The Compute Dissolution algorithm calculates the following:

Dissolved Amount: the amount of component that is dissolved at the time the sample was taken.

Dissolved Percent: the percent of the claimed amount of component dissolved at the time the sample was taken. The claimed amount is the amount the manufacturer claims is in the dosage form.

Following is the base equation the Compute Dissolution Algorithm uses. Variations on the formula are dependent on whether volume is replaced and/or if there is recirculation of media, depending on whether the sampler allows it.

It’s that easy!