Empower Tip: Questions from Our Readers

Question: How do you calculate impurity response when there are two APIs in the sample?

Another great question.

In this chromatogram we see two API peaks – API A and API B. We also see some impurity peaks, some related to API A and some related to API B. The goal is to calculate the %Area against the Main Component where the Main Component could be API A or API B.

On the Components tab of the Processing Method, we assign the CCompRef1 to be the API that corresponds to each impurity peak.

We create a custom peak field that calculates %Area against the Main Component with a simple formula using the area of the CCompRef1 peak.

Back in the Processing Method we go to the Impurity tab and set the Impurity Response to the name of the custom field.

We integrate/quantitate and see the Impurity Response calculated for the impurity peaks using the proper API.

It’s that easy!