Empower Tip: Review Window and the Processing Method

In this tip, we will kick off a 2-Part discussion on creating and using MS Spectral libraries.

Part 1: Learning how to create an MS Spectral Library.

Spectral libraries can be helpful in identifying peaks in sample chromatograms by taking the apex spectrum from a peak and comparing it to spectra of known compounds. We begin by creating the Library.

STEP 1 Start by bringing data with the known compounds into Review. Once the Method Set has been opened and applied, select ‘New Library’ from the Library menu. Enter a name for the ‘New Library’ and click ‘Create’.

First, deselect any spectra you do not want added to the Library using the ‘Select’ field in the Spectra table, thus leaving the spectra you do want to add to the Library. Select ‘Add to Library’ from the Library menu (Note: name of Library from Step 1 should appear).

Enter a name and any descriptive information and click ‘OK’.

Once you have added all the spectra from the channels of data to the Library, you can add ‘Name’ and ‘Library Name’ to your Spectra table. This makes it easy to distinguish between Library spectra and spectra from peaks in chromatograms.

You can modify the name of a spectrum in the Name field. Press enter and you will see a warning message, click ‘OK’.

Libraries live outside of the project in Configuration Manager. Therefore, when you back up your project you need to back up the Library separately. This is very easy to do: Go to the Configuration Manager and click ‘Libraries’ in the tree on the left. Click and drag your Library to ‘External Media’. If the Library is deleted from Empower, you can click and drag it back. The same applies to PDA Libraries.

It’s that easy!