Empower Tip: Empower User Question

In this tip, we will answer another question from an Empower user regarding the overlay of UV spectra.

Q: How do we compare UV spectra from different channels in a report?

Please Note: In this tip, we will overlay spectra from both channels of data at the same retention time.

Highlight the 3D channels, Right click and select ‘Preview/Publisher’.

When the ‘Open Report Method’ dialogue opens, select ‘Use the currently opened Report Method named Untitled’ and click ‘OK’.

Right-click in the empty space on the right side of the window and select ‘Method Properties’.

Under ‘Report Type’, select ‘Summary By All’ and click ‘OK’.

On the left side of the window, expand ‘PDA/MS’ groups, expand ‘Extracted Spectral Plots’ and double click on the group called ‘254nm Extraction Group’.

Right-click on the group and select ‘Extraction Plot Properties’.

On the ‘Chromatogram’ tab, delete ‘line 1’.

On the ‘Spectra’ tab make the changes as shown in Figure 8 and enter the appropriate ‘extraction time’ for the spectrum.

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On the ‘Overlay’ tab, select either ‘Make Stack Plot’ or ‘Overlay in Single Plot’.

On the ‘Legend’ tab, delete the ‘Plot Title’.

Expand ‘Sample’.

Double-click on ‘SampleName’.

You now have an overlay of spectra from two different 3D PDA channels at the same retention time.

It’s that easy!