Empower Tip: Review Window and the Processing Method

Let’s start by bringing two different 3D PDA channels into Review.

Extract a wavelength from the first channel, in this case I chose 254nm from the Extract Choices drop-down list. (There are multiple ways of doing this and I will cover this topic in a future tip.)

From the File menu select Save, Chromatogram.

Repeat steps 2 & 3 for the second channel.

Exit out of Review and on the channels tab in Browse Project, notice there are now the single wavelength channels we saved.

Bring the single wavelength channels into review.

Click the Overlay tool.

You can customize the overlay by right clicking in the plot and selecting Properties.

On the Overlay tab you can change the display from Overlay in Single Plot to Make Stack Plot and under Chromatogram Annotation, select All Chroms in Overlay.

On the Legend tab you can add the Sample Name and the Channel (which displays the wavelength). Select Display Legend on Screen. Then click OK.

We now have a modified overlay and both channels are labeled with the Sample Name and wavelength.

That is one way to do it. Here is another way.

Once the 3D channels have been processed you can overlay the chromatograms easily. On the Results tab select the results.

Bring the results to Review and click the Overlay tool.

Modify the properties of the overlay as described in steps 8-11 and once again you have an overlay with both channels labeled properly.

It’s that easy!