Empower Tip: Empower User Question Answered

In this tip, we will answer another Empower user question.

QUESTION:

How do I switch wavelengths during data acquisition with a UV detector?

Great question and a special thank you to Melissa Jones from Bausch Health for providing this question and content for this week’s tip.

Let’s see how it’s done!

There are two types of UV detectors we will consider.

Type 1: Allows wavelength switching using the Events tab in the Instrument Method. (This includes the 2487, 2489, ACQUITY TUV, 2998 and ACQUITY PDA)

For this example, I chose the ACQUITY TUV. On the ‘Channel A’ tab, enter the initial wavelength.

On the ‘Events’ tab, enter the time you want to change the wavelength and select ‘Wavelength’ from the event list.

Finally, enter the Parameter. In this example, the wavelength we want to switch to, and select ‘Channel A’.

For the next example, I chose the 2998 PDA. On the ‘2D Channels’ tab, select ‘Channel 1’ and enter the initial wavelength, along with the Resolution.

On the ‘Events’ tab, enter the time you want to change the wavelength and select ‘Wavelength’ from the event list.

Finally, enter the Parameter. In this example, the wavelength you want to switch to, and select ‘Channel 1’.

If you collect 3D data with the PDA (for example 210 – 400nm), you can derive a 2D Channel using Timed Wavelength post-run.

Type 2: Does not allow wavelength switching via the Instrument Method. In this case, we collect in dual wavelength mode and then post run derive a channel to switch from one channel to the other. (This applies to the Alliance iS TUV)

In the TUV Detector section, select ‘Wavelength’ mode and select ‘Dual’.

Set the wavelength for Wavelength A.

Set the wavelength for Wavelength B.

Once the data has been collected, you can derive a channel using the ‘Switch To’ function. Derive a channel and on the First (Only) Channel tab select ‘Channel A’ from the Channel list.

On the Second Channel tab, select ‘Switch To’ from the Operator list. Select ‘Channel B’ from the Channel list and enter the time you want to switch from Channel A to Channel B.

It’s that easy!