waters_connect 4.0.0 LTS Release Overview

waters_connect Base Kit comprises of platform Services, utility tools and core applications such as Hub, Administration, Scientific Library for general system use.

Versions released

waters_connect 4.0.0 LTS is compatible with Base Kit 4.0.

waters_connect Base Kit 4.0 includes core services and applications such as Services 7.12, Administration 2.10 and Hub 4.3.

Benefits

Key benefits in this kit include:

• Enhanced lab operational security through the following measures:

  • Implementation of anti-tampering mechanisms for installed binaries using Verify Files.
  • Update to the latest third-party components used in waters_connect platform services.
  • Replacement of the deprecated DES algorithm.
  • Ability to operate waters_connect with Windows Defender enabled
  • Support for Windows 11 Pro and LTSC 2024 24H2 versions for Workstation installs and Client PCs in a network deployment.
  • Support for Windows Server 2025 Standard for Server PC in a waters_connect network deployment with seamless OS upgrades from Windows Server 2019
• Increase lab operational efficiency by enabling a more scalable network of users with the capacity to activate up to 100 users for waters_connect network installs, including the ability to monitor active user sessions.

• Improved laboratory operational productivity and reduced cost of ownership with the following:

  • Support of up to 9 Xevo Tandem Quads (increased from up to 5) on a single network install.

  • Support for 1 Xevo MRT system on a network install (with Xevo MRT 1.3.0 driver).

• Enhanced understanding of data storage capacity through a traffic light indicator and maintenance recommendation tooltips in the waters_connect HUB for optimized product use and performance.

• Enhanced customer experience with user interface improvements in the waters_connect HUB, including the ability to fit and display more application icons and an at-a-glance view of the waters_connect product version.

• Improved waters_connect Application Programming Interface (API) data streaming performance to accelerate workflow application data generation for ion chromatograms and data-dependent acquisition switch tables.

• Improved product quality and stability by addressing high impact software anomalies such as:

  • Slow generation of verify files and IQ reports.
  • Data buffering issues when changing location and method location on used sample list in sample submission.
  • Performance degradation with analysis methods containing signature methods.
  • Hanging state of other sample lists when using the 'Abort all' option.
  • Corruption of PDF exports for large reports.
  • Inconsistent LND restart in the Device Management application.

(includes Sample Sub 2.6, Method Editor 3.12, LND 1.23)waters_connect Acquisition Kit comprises of Sample Submission, Method Editor and Laboratory Network Device applications. This kit enables data acquisition with Waters instrument systems used with waters_connect Quantitative analysis software, such as Xevo TQ-S cronos, Xevo TQ-S micro, Xevo TQ-XS, Xevo TQ Absolute and Xevo TQ Absolute XR, and/or accurate mass applications with Xevo G2-XS QTof, Xevo G3 QTof, Xevo MRT, BioAccord LC-MS System and ACQUITY RDa detector.

Versions released

waters_connect 4.0.0 LTS is compatible with Acquisition Kit 3.2, Acquisition Kit 3.1 and Acquisition kit 3.0

Acquisition Kit 3.2 is the latest release and includes waters_connect applications such as Sample Submission 2.8, Method Editor 4.3 and LND 1.24.

Acquisition Kit 3.1 includes waters_connect applications such as Sample Submission 2.7, Method Editor 4.1 and LND 1.24.

Acquisition Kit 3.0 includes waters_connect applications such as Sample Submission 2.6, Method Editor 4.0, and LND 1.23.

Benefits

Key Benefits in Acquisition Kit 3.2 include:

• Extended the capabilities of your instrument systems configuration with the introduction of support for the ACQUITY FLR Detector within the Sample Submission and Method Editor applications.

• Increased productivity for the sample submission area by allowing users to define vertical or horizontal vial priority when filling a series in a sample plate.

Key benefits in Acquisition Kit 3.1 include:

• Increased productivity for the acquistion workflow with key improvements for the sample submission area such as:

  • Add and modify entries in the sample list from within the sample plate layout.
  • Default the selected plate type to last plate type saved with a sample list.
  • Allow the user to submit a sample list as acquire only assay with waters_connect application processing settings.
  • Maintain the sample list name to match the processing application result set name when launching Sample Submission from a waters_connect workflow application.
  • Use a sample list as a template to run new acquire and process analyses.

• Expanded capabilities of your instrument systems inlets with support for two-dimensional liquid chromatography (2D-LC) Trap & Elute experiments for waters_connect for Quantitation with our Tandem Quad instruments within the sample submission and acquisition method editor workflow. Increase your assay sensitivity with 2D-LC Trap & Elute by removing background noise, matrix interferences, and co-eluting components from the target analyte of interest.

Key benefits in Acquisition Kit 3.0 include:

• Expanded capabilities of your instrument systems inlets and sources with support for GC-HRMS workflow using the Xevo G3 QTof, now compatible with:
  • Agilent 8890 GC system.
  • Agilent 7693A Automatic Liquid Sampler system.
  • CTC PAL3 Series 2 Autosampler system.
  • Waters Atmospheric Pressure Gas Chromatography (APGC) source.
  • waters_connect sample submission for acquisition and UNIFI for data processing, in workstation deployment.
• Expanded waters_connect for Quantitation instrument systems configuration with addition of the Xevo TQ Absolute XR mass spectrometer.

waters_connect LC-MS Toolkit enables data interrogation outside of the defined workflows and provides flexibility to the software that is needed for more advanced use.

Versions released

waters_connect 4.0.0 LTS is compatible with LC-MS Toolkit 1.17 and LC-MS Toolkit 1.16.

waters_connect LC-MS Toolkit 1.17 is the latest release.

Benefits

Key Benefits in LC-MS Toolkit 1.17 include:

• Increased performance and speed to results in the LC-MS Toolkit application when handling up to 100 concurrent Tandem Quad MRM spectra in both workstation and network installs.

Key benefits in LC-MS Toolkit 1.16 include:

• Increased data clarity and speed to results with data view and interrogation enhancements in the LC-MS Toolkit application, such as:
  • Manual integration of a chromatogram.
  • Performance improvements for XIC and DDA switch table generation.
  • Improved advanced extract tool.

waters_connect Data Convert enables increased data compatibility with third-party software applications via conversion of MS, MS/MS, DDA and MSE data into .mzML format.

Versions released

waters_connect 4.0.0 LTS is compatible with Data Convert 3.0

Benefits

Key benefits in this application include:

• Improved data clarity and usability when converting data to generic file format (.mzML) with enhancements in the Data Convert application, including:
  • Component detection (peak picking, isotope deconvolution and, where appropriate, CCS calculation), excluding when acquire & export is used with ACQUITY RDa.
  • Advanced user settings for arguments definition.
  • Storage space capacity notifications.

waters_connect MS Quan is a quantitative analysis software available on our core Xevo tandem quadrupole family of mass spectrometers. It enables routine small-molecule quantitation and accelerates quantitation data processing and review steps with exception-focused review and easy-to-view chromatogram windows for multiple samples.

waters_connect 4.0.0 LTS is compatible with MS Quan 2.2, MS Quan 2.1, MS Quan 2.3 and MS Quan 2.4.

waters_connect MS Quan 2.4 is the latest release.

Key Benefits in MS Quan 2.4 include:

• Expanding MS Quan application capabilities with a focus on enhancements for PFAS analysis, improved processing efficiency, data display and reporting, such as:

  • Recovery calculations based on concentration as defined in EPA methods 533 and 537.1

  • Peak symmetry measurement to assist with Demonstrations of Capability as in EPA 537.1 and evaluation of chromatographic changes

  • Ability to use existing calibration curves to reduce time and cost associated with running standards

  • Fast and efficient deletion of peaks from one or more chromatogram directly from the PI page

  • Quicker set up and import of methods using summed transitions

Key Benefits in MS Quan 2.3 include:

• Expanding MS Quan application capabilities with focus on significant usability and productivity enhancements including:

  • Ability to sum MRM transitions for quantitation

  • Support for Pharmacopeia S/N calculations

  • Ability to include multiple sample factors to account for dilution/concentration, sample weights etc.​

  • Streamlining the manual integration​ workflow when re-integration is necessary

  • Option to view previous integrations

  • Inclusion of peak height in Data Summary

Key Benefits in MS Quan 2.2 include:

• Expanding MS Quan application capabilities with focus on regulatory compliance, processing and reporting enhancements including:

  • Flexible recovery limits for internal standards (IS) to allow set minimum and maximum values.

  • Composite IS definition to create IS’s which are combinations of multiple IS’s.

  • New chart showing recovery deviation for IS.

  • Flag IS response deviations based on mean values calculated based on specific sample types.

  • Report in PDF format results set audit trail details.

  • Reporting improvements such as separation of qualifier ions in pdf report, print to pdf processing method details, font size selector.

  • MS Quan result sets application programming interface end points to facilitate integration with 3^rd party software such as LIMS.

Key benefits in MS Quan 2.1 include:

• Expanding MS Quan application capabilities with focus on impurity processing, including:
  • Optical data processing (TUV & PDA).
  • Recovery calculations.
  • Relative standard error.
  • Lock data on signing reports.
  • Per-injection reports including overlays.
  • Simple exceptions in report tables.

waters_connect UNIFI enables the execution of accurate mass experiments ranging from Small Molecules to Biologics. Moreover, it provides access to core applications such as System Console, Device Management, Explorer, and Qualification for general system use.

waters_connect 4.0.0 LTS is compatible with UNIFI 3.15.1 and UNIFI 3.14.1.

waters_connect UNIFI 3.15.1 is the latest release.

Key Benefits in UNIFI 3.15.1 this application include:

• Improved speed to results via a novel peak processing mechanism that in combination with waters_connect Lenovo P5 HRMS Performance PC offers users running accurate mass studies an improvement in processing time of up to 91% vs. previous system configurations (e.g. with waters_connect 3.2.0 LTS and Lenovo P520 4-cores PC).

Key benefits in UNIFI 3.14.1 include:

• Achieve quicker decisions using UNIFI Accurate Mass workflows by reducing the time needed for generating reports and opening analysis methods that incorporate electronic signature methods.
• Improved data clarity when importing MassLynx data by including more information in imported sample lists and sample sets with new fields such as subject, submitter, MassLynx user and sample dilution.

The waters_connect Pattern Analysis application increases data clarity in complex assays and enhances the confidence in identifying contaminants, such as polymers, PFAS and PCBs.

Versions released

waters_connect 4.0.0 LTS

is compatible with Pattern Analysis 1.0.

Benefits

Key benefits in this application include:

• Leverage the unique mass spectral properties of chemicals to resolve them from matrix background.
• Data review workflow with interactive plots to aid in compound classification.
• Confidence in identifying emerging exogenous contaminants.
• Development of a comprehensive library for targeted comparison.

waters_connect Peptide Multi-Attribute Method (MAM) enables monitoring of biopharmaceutical product quality attributes (PQA) at the peptide level.

Versions released

waters_connect 4.0.0 is compatible with Peptide MAM 1.8.2

Benefits

Key Benefits in Peptide MAM 1.8.2 include:

• General maintenance release

• Defect fixes

waters_connect Intact Mass enables biologics mass confirmation and purity determination of oligonucleotides, peptides, protein and conjugates.

Versions released

waters_connect 4.0.0 LTS is compatible with Intact Mass 1.9.

Benefits

Key Benefits in this application include:

• Automated Calculations of DAR and Drug Distribution for Conjugated Molecule Analysis - Users can now deploy a BioAccord System, Xevo G3 QTof, or Xevo MRT QTof to analyze conjugated molecules, specifying the conjugate and its mass in the INTACT Mass data processing parameters, and have the calculation of Drug Antibody Ratio and distribution of conjugate forms automatically performed from the acquired data.

• Controls for manual adjustments of chromatographic integration - Users can now manually adjust chromatographic peak integration and associated peak identification results and use those reintegrated peaks to direct the processing of mass spectral data.

• Sample List Entry of Chemical Formula - User can now specify a ‘Chemical formula for deconvolution’ that will be used to refine the deconvolution isotope model, improving deconvolution mass accuracy and quantification.

• Updated Sample List Column ‘Expected Masses’ changed to ‘Expected Masses and/or Chemical Formulas’: Users now can enter targets as numeric masses, as chemical formulas, or a mixture of both.

• New Method option within ‘Specify calculation parameters’, allowing the user more control over the ‘Main Peak Retention Time Window’ to improve main peak identification in Optical Only and Variant Analysis workflows

• Bioprocessing workflow: Additional sample list columns for Time Point and Dilution Factor that facilitates processing time series trending data (in bioprocess monitor or in third party solutions) and dilution factor to enable clearer results visualization and trending.

waters_connect Synthetic Library supports the Confirm Sequence and Map Sequence applications for oligo sequencing and mapping workflows.

Versions released

waters_connect 4.0.0 is compatible with Synthetic Library 2.0.

Benefits

Key Benefits in Synthetic Library 2.0 include:

• Support for oligo mapping workflow - new enzymes and polynucleotides sections

• Multiple library support including user access controls

waters_connect Confirm Sequence enables streamlined oligonucleotide sequence confirmation and impurity characterization workflow.

Versions released

waters_connect 4.0.0 is compatible with Confirm Sequence 2.0.

Benefits

Key Benefits in Confirm Sequence 2.0 include:

• Compatibility with Synthetic Library 2.0.0

• Maximum charge state support extended to -20

waters_connect Map Sequence enables accurate mass mapping of digested oligonucleotides.

Versions released

waters_connect 4.0.0 is compatible with Map Sequence 2.0.

Benefits

Key Benefits in Map Sequence 2.0 include:

• Supported by Synthetic Library for sequence entry and experimental planning

• Oligo mapping and sequence confirmation using LCMSE precursor and fragment data

• Interactive annotated chromatogram

• Interactive coverage map for simple visualization of sequence coverage and navigation to supporting data

• Drill-down to fragment analysis

• Dynamic pass-fail filtering and acceptance criteria for investigation and curation of results

• Integrated reporting

The waters_connect Bioprocess Monitor application enables routine, in-process monitoring of spent media components and product titer via absolute and relative quantification using LC/UV, LC/MS & LC-UV/MS techniques.

Versions released

waters_connect 4.0.0 LTS is compatible with Bioprocess Monitor 1.0.

Benefits

Key benefits in this application include:

• Near-real time quantitative results for in-process samples with a streamlined sample analysis workflow and automated processing.
• Straight to the answer you need with a simplified results review.
• Confidence in the data with robust data processing and ability to review by exception.
• Statistical evaluation of the data with connectivity with third-party software JMP.
• Full integration with Bioprocess Walk-Up Solutions and Sartorius Ambr data interface.

Quantitative analysis software available on our core Xevo tandem quadrupole family of mass spectrometers, enabling laboratories to process large sample batches quickly and easily, significantly reducing the time spent on data review. Maximize your productivity with a compliant-ready workflow for analytical methodologies ranging from PFAS to genotoxic impurity analysis.

Bioprocess accurate mass applications for process (spent cell culture media) and product quality attribute monitoring with the BioAccord LC-MS system and Bioprocess Walk-Up Solutions.

Bioprocess accurate mass applications for process (spent cell culture media and product titer) and product quality attribute monitoring including the Bioprocess Walk-Up Solution.

Accurate mass-based solutions for targeted and non-targeted screening of small molecules, offering versatile qualitative and quantitative analysis of compounds such as pesticides, drugs of abuse, metabolites, impurities, extractables and leachables, and identification of contaminants like polymers, PFAS, and PCBs in complex samples.

Accurate mass-based metabolomics, lipidomics and metabolite identification solutions with integration with 3rd Party software (via .mzML and/or via API).

Operating System
Windows

Requirements

• OS language: English (US), Japanese (Japan).
• Windows images provided with waters_connect PCs include Windows IoT Enterprise licensing which enables extended support on the operating system end of service over the standard edition. View more information on Windows releases.

Versions Supported
Workstation PC

• Windows 10 Enterprise LTSC 2021 edition (version 21H2)
• Windows 10 Enterprise IoT LTSC 2021 edition (version 21H2)
• Windows 11 Enterprise LTSC 2024 edition (version 24H2)
• Windows 11 Enterprise IoT LTSC 2024 edition (version 24H2)
• Windows 11 Pro version 24H2

Operating System
Windows

Requirements

• OS language: English (US), Japanese (Japan)
  • Note: for Client PCs only the software deployment is supported on European and Japanese language versions of Windows.  However, Hub is displayed in English (US) or Japanese (Japan) only.
• Windows images provided with waters_connect PCs include Windows IoT Enterprise licensing which enables extended support on the operating system end of service over the standard edition. View more information on Windows releases.

Versions Supported