waters_connect 4.2.0 Release Overview

waters_connect Base offering comprises of platform Services, utility tools and core applications such as Hub, Administration, Scientific Library for general system use.

Versions released

waters_connect 4.2.0 includes core services and applications such as Services 7.14, Administration 2.12 and Hub 4.3.

Benefits

Key Benefits in this kit include:

• Increased lab operational efficiency through the support of automatic data transfer to a desired storage location & data removal from the production install, including:

  •  Define admin policies to schedule by date/time data transfer and removal.

  • Flexible data transfer destination options, such as customer cloud storage.

  • Compatibility with data and records generated via workflow applications such as MS Quan, UNIFI, Intact Mass, Bioprocess Monitor, Peptide MAM, MS Toolkit and Data Convert (note: consult the application tables below for version compatibility).

  • Waters recommends to restore any remaining OSM data, and archive it using the new automatic data transfer mechanism.

• Improved laboratory operational productivity and reduced cost of ownership with the support for up to 2 Xevo MRTs in a mixed network with Tandem Quadrupoles and other HRMS systems.
• Improved acquire data service performance, achieving up to 50% faster data access by transitioning the Data Reader component to Microsoft .Net 8.0
• Improved product quality and stability by addressing high impact software anomalies.

waters_connect Acquisition Kit comprises of Sample Submission, Method Editor and Laboratory Network Device applications. This kit enables data acquisition with third party GC instruments and Waters instrument systems used with waters_connect Quantitative analysis software, such as Xevo TQ-S cronos, Xevo TQ-S micro, Xevo TQ-XS, Xevo TQ Absolute and Xevo TQ Absolute XR & accurate mass applications with Xevo G2-XS QTof, Xevo G3 QTof, Xevo MRT, Xevo CDMS, BioAccord LC-MS System and ACQUITY RDa detector.

Versions released

waters_connect 4.2.0 is compatible with Acquisition Kit 3.3 & Acquisition Kit 3.2.

Acquisition Kit 3.3 is the latest release and includes waters_connect applications such as Sample Submission 2.9, Method Editor 4.4 and LND 1.25.

Acquisition Kit 3.2 includes waters_connect applications such as Sample Submission 2.8, Method Editor 4.3 and LND 1.24.

Benefits

Key Benefits in Acquisition Kit 3.3 include:

• Increased productivity for the acquisition workflow with key improvements, such as:

  • Allow the user to acquire data with custom sample trays for ANSI-96 or ANSI-384 plates via definition of well plate depth and height.

  • View real time plots for Xevo CDMS instrument.

  • Enablement work for ACQUITY UPLC Sample Manager auto addition to automate the preparation of standard solution (needs ACQUITY LC DP 3.9.0).

  • Load ahead functionality support for CTC PAL autosampler.

  • Third party LC and GC driver support for deployment in a waters_connect Network install.

  • Improved product quality and stability by addressing high impact software anomalies such as data buffering & transfer when sample set location was moved.

Key Benefits in Acquisition Kit 3.2 include:

• Extended the capabilities of your instrument systems configuration with the introduction of support for the ACQUITY FLR Detector in 2D mode within the Sample Submission and Method Editor applications.

• Increased productivity for the sample submission area by allowing users to define vertical or horizontal vial priority when filling a series in a sample plate.

waters_connect LC-MS Toolkit enables data interrogation outside of the defined workflows and provides flexibility to the software that is needed for more advanced use.

Versions released

waters_connect 4.2.0 is compatible with LC-MS toolkit 1.18

Benefits

Key Benefits in LC-MS Toolkit 1.18 include:

• Increased data clarity with data view and interrogation enhancements, such as:

  • Step-through chromatogram navigation to enable users to walk through chromatographic peaks one data point at a time.
  • Display and process FLR chromatograms for improved fluorescence-based data interpretation.
  • Allow users to review data using the IONIS method for advanced biologics interrogation workflows.
  • Generate Survey Scan spectra directly from the DDA Switch Table for streamlined spectral review.

• Improved performance and faster results for DDA Switch Tables via an intelligent caching mechanism.

• Compute theoretical Collision Cross Section (CCS) values to support structural analysis.

• Application compatibility with data management.

The Data Convert application for waters_connect enables increased data compatibility with third-party software applications via conversion of Optical data, MS, MS/MS, DDA and MSE data into .mzML format.

Versions released

waters_connect 4.2.0 is compatible with DATA Convert 4.0.

Benefits

• Improved data compatibility and workflow flexibility to boost interoperability and usability when converting analytical data to the generic .mzML format with support for:

  • PDA and TUV data.

  • HDMSE (ion mobility) data.

  • Aligned DIA

  • MRM data (Tandem Quad and Tof MRM)

waters_connect MS Quan application is a quantitative analysis software available on our core Xevo tandem quadrupole family of mass spectrometers. It enables routine small-molecule quantitation and accelerates quantitation data processing and review steps with exception-focused review and easy-to-view chromatogram windows for multiple samples.

Versions released

waters_connect 4.2.0 is compatible with MS Quan 2.4.

Benefits

Key Benefits in MS Quan 2.4 include:

• Expanding MS Quan application capabilities with a focus on enhancements for PFAS analysis, improved processing efficiency, data display and reporting, such as:

  • Recovery calculations based on concentration as defined in EPA methods 533 and 537.1
  • Peak symmetry measurement to assist with Demonstrations of Capability as in EPA 537.1 and evaluation of chromatographic changes
  • Ability to use existing calibration curves to reduce time and cost associated with running standards
  • Fast and efficient deletion of peaks from one or more chromatogram directly from the PI page
  • Quicker set up and import of methods using summed transitions

• Application compatibility with data management.

waters_connect UNIFI application enables the execution of accurate mass experiments ranging from Small Molecules to Biologics. Moreover, it provides access to core applications such as System Console, Device Management, Explorer, and Qualification for general system use.

Versions released

waters_connect 4.2.0 is compatible with UNIFI 3.16.

Benefits

Key Benefits in UNIFI 3.16 include:

• Increase Lab operational efficiency with performance improvements, including:

  • Opening the system console application in a Network install.

  • Processing complex analytical datasets such as for Forensic Toxicology workflows.

• Reduced hardware load by preventing duplication of stored data during automated exports.

• Improved product quality and stability by addressing high impact software anomalies, such as:

  • Out of memory when processing with keep manual integration.

  • Failures when processing sample set larger than 64 injection with a forensic tox method.

  • Missing information when creating an acquire only method.

• Application compatibility with data management.

The waters_connect Pattern Analysis application increases data clarity in complex assays and enhances the confidence in identifying contaminants, such as polymers, PFAS and PCBs.

Versions released

waters_connect 4.2.0 is compatible with Pattern Analysis 1.1.

Benefits

Key Benefits in Pattern Analysis 1.1 include:

• Ability to process MS data that has been acquired together with PDA data.

• Introduce the ability to Create, Clone, Delete and Select Pattern Analysis methods.

• Ability to specify a m/z range for data processing and detect monomer sequences based on the specified mass range.

• Components can be banked and unbanked from the Component List table.

• Enable library searching to be performed with respect to retention time in addition to mass.

• Filtered components can be exported as a Pattern Analysis library.

• Ability to change the default assignment when multiple targets are assigned to a component.

waters_connect Peptide Multi-Attribute Monitoring (MAM) application enables monitoring of biopharmaceutical product quality attributes (PQA) at the peptide level.

Versions released

waters_connect 4.2.0 is compatible with Peptide MAM 1.8.2.

Benefits

Key Benefits in Peptide MAM 1.8.2 include:

• "Find related data" option on an analysis in Explorer to discover reports
  created from that analysis.

• Launch LC-MS Toolkit directly from the New Peak Detection and Monitored
  Attributes steps, giving you quick and easy access to detailed injection information.

• Application compatibility with data management.

waters_connect Intact Mass application enables biologics mass confirmation and purity determination of oligonucleotides, peptides, protein and conjugates.

Versions released

waters_connect 4.2.0 is compatible with Intact Mass 1.9.

Benefits

Key Benefits in Intact Mass 1.9 include:

• Automated Calculations of DAR and Drug Distribution for Conjugated Molecule Analysis - users can now deploy a BioAccord System, Xevo G3 QTof, or Xevo MRT QTof to analyze conjugated molecules, specifying the conjugate and its mass in the INTACT Mass data processing parameters, and have the calculation of Drug Antibody Ratio and distribution of conjugate forms automatically performed from the acquired data.

• Controls for manual adjustments of chromatographic integration - users can now manually adjust chromatographic peak integration and associated peak identification results and use those reintegrated peaks to direct the processing of mass spectral data.

• Sample List Entry of Chemical Formula - users can now specify a ‘Chemical formula for deconvolution’ that will be used to refine the deconvolution isotope model, improving deconvolution mass accuracy and quantification.

• Updated Sample List Column ‘Expected Masses’ changed to ‘Expected Masses and/or Chemical Formulas’ - users now can enter targets as numeric masses, as chemical formulas, or a mixture of both.

• New Method option within ‘Specify calculation parameters’, allowing the user more control over the ‘Main Peak Retention Time Window’ to improve main peak identification in Optical Only and Variant Analysis workflows.

• Bioprocessing workflow: Additional sample list columns for Time Point and Dilution Factor that facilitates processing time series trending data (in bioprocess monitor or in third party solutions) and dilution factor to enable clearer results visualization and trending.

• Application compatibility with data management.

waters_connect Confirm Sequence application enables streamlined oligonucleotide sequence confirmation and impurity characterization workflow.

Versions released

waters_connect 4.2.0 does not support Confirm Sequence.

waters_connect Map Sequence application enables accurate mass mapping of digested oligonucleotides.

Versions released

waters_connect 4.2.0 does not support Map Sequence.

The waters_connect Bioprocess Monitor application enables routine, in-process monitoring of spent media components and product titer via absolute and relative quantification using LC/UV, LC/MS & LC-UV/MS techniques.

Versions released

waters_connect 4.2.0 is compatible with Bioprocess Monitor 1.1.1.

Benefits

Key benefits in Bioprocess Monitor 1.1.1 include:

• Application compatibility with data management.

The waters_connect CDMS Toolkit application empowers customers to unlock the full potential of Xevo CDMS by enabling access to real-time data acquisition, native analysis, and advanced visualization, streamlining workflows and accelerating insights into large, complex biomolecules.

Versions released

waters_connect 4.2.0 is compatible with CDMS Toolkit 1.0.

Benefits

Key benefits in CDMS Toolkit 1.0 include:

• Enables native analysis of large protein assemblies and multi-subunit complexes, including tools for assessing molecular weight, purity, and heterogeneity.

• Real-time display of mass, mass-to-charge, and charge spectra, along with corresponding 2-dimensional heatmaps.

• Raw data review for pre-acquired and live acquisition.

• Relative quantitation analysis to determine quantities of user defined targets.

• Application compatibility with data management.

The MS Imaging application with waters_connect control enables customers to generate molecular spatial information directly from surfaces using the DESI XS Xevo MRT MS system solution. The integration of DESI XS with Xevo MRT MS enables high spatial resolution, label-free molecular imaging directly from sample surfaces with minimal preparation, offering highly sensitive MS Imaging at speeds of up to 100 Hz, with sub-ppm mass accuracy, making it ideal for applications in biomedical research, drug distribution studies, and forensic analysis.

Versions released

waters_connect 4.2.0 is compatible with MS Imaging 1.0 in combination with Xevo MRT v1.5.0.

Benefits

Key benefits in MS Imaging 1.0 include:

• Application specific tuning parameters for MS Imaging.

• Control for DESI XS, ACQUITY DESI uBSM and Xevo MRT MS under waters_connect.

• Data generation for processing with Waters HDI 1.9.

• Microscope mode workflow for targeted high spatial resolution MS Imaging as well as targeted MS/MS.

• Export as imzML or .RAW for 3rd party processing (e.g. Scils or Lipostar MSI).

Quantitative analysis software available on our core Xevo tandem quadrupole family of mass spectrometers, enabling laboratories to process large sample batches quickly and easily, significantly reducing the time spent on data review. Maximize your productivity with a compliant-ready workflow for analytical methodologies ranging from PFAS to genotoxic impurity analysis.

Biopharmaceutical accurate mass applications for protein-based therapeutics, vaccines, and nucleic acid-based therapeutics.

Bioprocess accurate mass applications for process (spent cell culture media) and product quality attribute monitoring with the BioAccord LC-MS system and Bioprocess Walk-Up Solutions.

Achieve precise mass measurement of challenging large and highly heterogeneous biologics including viral vectors, virus-like particles (VLPs), RNA, and protein complexes that were previously out of reach for mass characterization.

Accurate mass-based solutions for targeted and non-targeted screening of small molecules, offering versatile qualitative and quantitative analysis of compounds such as pesticides, drugs of abuse, metabolites, impurities, extractables and leachables, and identification of contaminants like polymers, PFAS, and PCBs in complex samples.

Accurate mass-based metabolomics, lipidomics and metabolite identification solutions with integration with 3rd Party software (via .mzML and/or via API)

Accurate mass-based metabolomics, lipidomics and metabolite identification solutions with integration with 3rd Party software (via .mzML and/or via API)

Operating System: Windows

Requirements

• OS language: English (US), Japanese (Japan).

• Windows images provided with waters_connect PCs include Windows IoT Enterprise licensing, which enables extended support on the operating system end of service over the standard edition. For more information on Windows releases, visit https://learn.microsoft.com/en-us/windows/release-health/

Versions Supported
Workstation PC

• Windows 10 Enterprise LTSC 2021 edition (version 21H2)

• Windows 10 Enterprise IoT LTSC 2021 edition (version 21H2)

• Windows 11 Enterprise LTSC 2024 edition (version 24H2)

• Windows 11 Enterprise IoT LTSC 2024 edition (version 24H2)

• Windows 11 Pro version 24H2

Operating System: Windows

Requirements

• OS language: English (US), Japanese (Japan). Note: for Client PCs only the software deployment is supported on European and Japanese language versions of Windows. However, Hub is displayed in English (US) or Japanese (Japan) only.

• Windows images provided with waters_connect PCs include Windows IoT Enterprise licensing, which enables extended support on the operating system end of service over the standard edition. For more information on Windows releases, visit https://learn.microsoft.com/en-us/windows/release-health/

Versions Supported