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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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System preparation

Installing the column

Installing the column
Topic LCI-USG-0007, Last Updated 11/21/2025

Install the column in the CHC prior to running samples.

Fittings and column clips are designed to be intuitive when installing a column in the Alliance iS Column Heater/Cooler (CHC).

Notes:
  • Power-on the system before installing the column to ensure proper RF column identification when the CHC door is closed. See Powering-on the system.
  • Waters BioSuite columns and some non-Waters columns may leak when connected with the tool-free fitting. In this case, install an adapter between the column and the tool-free fitting. See Installing the tool-free fitting adapter.
  1. From the touchscreen, tap MAINTAIN > Replace Components > Replace Column and follow the on-screen prompts.
  2. Open the column compartment door.
  3. Move the lower column clip as necessary to match the size of the column.
  4. Remove the plugs from the inlet and outlet ends of the column.
  5. Orient the column so that the outlet faces up (see the arrow on the column) and the inlet faces down.
  6. Screw the fittings on the column compartment tubing finger-tight to the column inlet and outlet.
  7. Insert the column into the upper and lower clips so that the clip grasps the exposed threads on the fitting.
    Figure 1: Column seated in column clip
  8. Close the column compartment door.
    • If an eConnect column is installed, the system reads the eConnect column tag information and displays it on the touchscreen.
    • If you suspect that the column information displayed is incorrect, inspect the column installation and ensure that the column includes an eConnect tag.
    • To re-read the eConnect tag, open and close the column compartment door, or, with the column compartment door closed, tap READ TAG.
  9. Follow the remainder of the on-screen prompts and tap DONE.
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