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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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Method management

Transferring methods

Transferring methods
Topic LCI-USG-0123, Last Updated 05/19/2025
The Alliance iS HPLC System can produce results comparable to those achieved on many other HPLC systems. However, when moving a regulated method from one system to another, typically no modifications to the instrument method can be made. For example, the column type (diameter) specified for a transferred method cannot be changed. Even with the same column type, results may not always be identical. If there is a significant difference in retention time, the dwell volume can be compensated by adjusting the gradient start relative to the injection. Extra-column effects can also be modified.
Note: When using Empower 3 FR4 through 3.8.0, the Run Samples window will display Alliance iS methods not compatible with the system being used. If the user attempts to run an incompatible method, the system status indicator will display RUN FAILED.

See the Dwell Volume and Extra-Column Volume: What Are They and How Do They Impact Method Transfer (720005723EN) white paper on www.waters.com.

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