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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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Daily routine analysis

Installing or replacing the column

Installing or replacing the column
Topic LCI-USG-0191, Last Updated 05/19/2025
Install or replace the column required for the method after placing the filled solvent bottles on the tray so that old mobile phase cannot pass through the new column.
Notes:
  • Power-on the system before installing or replacing the column to ensure proper RF column identification when the CHC door is closed. See Powering-on the system.
  • Waters BioSuite columns and some non-Waters columns may leak when connected with the tool-free fitting. In this case, install an adapter between the column and the tool-free fitting. See Installing the tool-free fitting adapter.
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